Inexpensive isolation of beta-D-glucopyranosidase from alpha-L-arabinofuranosidase,alpha-L-rhamnopyranosidase,and o-acetylesterase |
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| Authors: | Barbagallo Riccardo N Spagna Giovanni Abbate Cristina Azzaro Giuseppe Palmeri Rosa |
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| Institution: | (1) Biotechnology Group from the Department of Horticulture, Floriculture, Arboriculture and Agroindustrial Technology (DOFATA), University of Catania, Via S. Sofia 98, 95123 Catania, Italy |
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| Abstract: | β-d-Glucopyranosidase (βG, EC 3.2.1.21) has been isolated from some collateral activities, α-l-arabinofuranosidase (Ara, EC3.2.1.55), α-l-rhamnopyranosidase (Rha, EC 3.2.1.40), and o-acetylesterase (Est, EC 3.1.1.53), using a commercial enzyme preparation and a simple method economically sustainable for
the food industry. The procedure comprises precipitation of extraneous substances by adding ethanol and CaCl2, ultrafiltration, and adsorption, first on bentonite and then on chitosan. The results obtained were the complete isolation
of βG from the above-mentioned activities, a drastic reduction in extraneous compounds, such as brown substances and polysaccharides,
and a slight increase in purification. |
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| Keywords: | β d-Glucopyranosidase isolation" target="_blank">-d-Glucopyranosidase isolation α l-arabinofuranosidase" target="_blank">-l-arabinofuranosidase α l-rhamnopyranosidase" target="_blank">-l-rhamnopyranosidase o-acetylesterase brown substances and polysaccharides |
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