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Non-radioactive determination of PTH and dabsyl phosphoamino acids by capillary electrophoresis |
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| Authors: | M Heber Chr Liedtke H Korte E Hoffmann-Posorske A Donella-Deana L A Pinna J Perich E Kitas R B Johns H E Meyer |
| Institution: | (1) Institut für Physiologische Chemie, Abteilung Biochemie Supramolekularer Systeme, Protein-Sequenzlabor, MA 2/143, Ruhr-Universität Bochum, 4630 Bochum, Germany;(2) Dipartimento di Chimica Biologica, University di Padova, Via Trieste 75, 35121 Padova, Italy;(3) Department of Organic Chemistry, University of Melbourne, 3052 Parkville, Victoria, Australia |
| Abstract: | Summary Capillary electrophoresis is a novel technique in the non-radioactive determination of phosphoamino acids. The main advantage of the method presented is the high selectivity and the ability to separate all phosphoamino acid derivatives. Non-radioactive determination of PTH or dabsyl phosphoamino acids by capillary electrophoresis provides a fast and simple screening procedure for all O-phosphorylated amino acids in protein and peptides in the low picomolar range. |
| Keywords: | Capillary electrophoresis Phosphoserine phosphothreonine phosphotyrosine PTH-phosphoamino acids Dabsyl phosphoamino acids |
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