High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum |
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Authors: | Schellen A Ooms B van Gils M Halmingh O van der Vlis E van de Lagemaat D Verheij E |
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Institution: | Spark Holland BV, P.O. Box 388, 7800 AJ Emmen, The Netherlands. |
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Abstract: | The feasibility of high throughput on-line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns. |
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