Increased phosphatidylcholine (16:0/16:0) in the folliculus lymphaticus of Warthin tumor |
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Authors: | Qian He Yoshinori Takizawa Takahiro Hayasaka Noritaka Masaki Yukiko Kusama Jiping Su Hiroyuki Mineta Mitsutoshi Setou |
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Affiliation: | 1. Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Rd, Nanning, 530021, China 2. Department of Otorhinolaryngology/Head & Neck Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan 3. Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan 4. Department of Diagnostic Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan
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Abstract: | Warthin tumor (War-T), the second most common benign salivary gland tumor, consists mainly of neoplastic epithelium and lymphoid stroma. Some proteins and genes thought to be involved in War-T were evaluated by molecular biology and immunology. However, lipids as an important component of many tumor cells have not been well studied in War-T. To elucidate the molecular biology and pathogenesis of War-T, we investigated the visualized distribution of phosphatidylcholines (PCs) by imaging mass spectrometry (IMS). In our IMS analysis of a typical case, 10 signals were significantly different in intensity (p?0.01) between the War-T and non-tumor (Non-T) regions. Five specific PCs were frequently found in the War-T regions of all of the samples: [PC (16:0/16:0)?+?K]+ (m/z 772.5), [PC (16:0/20:4)?+?K]+ (m/z 820.5), [PC (16:0/20:3)?+?K]+ (m/z 822.5), [PC (18:2/20:4)?+?K]+ (m/z 844.5), and [PC (18:0/20:5)?+?K]+ (m/z 846.5). PC (16:0/16:0) was increased specifically in the folliculus lymphaticus of War-T lymphoid stroma, suggesting a different metabolism. Localization of PC (16:0/16:0) might reflect inflammation activity participating in the pathogenesis of War-T. Thus, our IMS analysis revealed the profile of PCs specific to the War-T region. The molecules identified in our study provide important information for further studies of War-T pathogenesis. |
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