High-resolution study of nuclear magnetic relaxation dispersion of purine nucleotides: effects of spin-spin coupling |
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Authors: | Kiryutin Alexey Ivanov Konstantin Yurkovskaya Alexandra Vieth Hans-Martin |
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Institution: | Institut für Experimentalphysik, Freie Universit?t Berlin, Arnimallee 14, 14195 Berlin, Germany. |
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Abstract: | By combining magnetic field cycling in the range from 0.1mT to 7T with high-resolution NMR detection the T(1) relaxation dispersion (nuclear magnetic relaxation dispersion (NMRD)) of protons in the nucleotides adenosine mono-phosphate and guanosine mono-phosphate was measured in a site-specific way. While at high field the individual spins have distinctly different T(1) times, their scalar spin-spin interaction fulfills at low field the condition of strong coupling and leads to convergence of their T(1) dispersion curves. In addition, the spin-spin coupling can lead to oscillatory components in the relaxation kinetics traceable to a coupling between spin polarization and coherence in the relaxation process. As a consequence the NMRD curves do not directly reflect the spectral density function of the motional processes, but the effects of motion and spin coupling must be separated for a reliable evaluation. A theoretical approach is described allowing such an analysis. |
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