X-ray structure analysis of free-standing lipid membranes facilitated by micromachined apertures |
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Authors: | Beerlink André Wilbrandt P-J Ziegler E Carbone D Metzger T H Salditt Tim |
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Institution: | Institut für R?ntgenphysik, Georg-August-Universit?t G?ttingen, Friedrich-Hund-Platz 1, D-37077 G?ttingen, Germany. |
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Abstract: | Silicon and Teflon substrates have been structured by wet etching and a focused ion beam (FIB) to obtain very defined, clean apertures. Planar, free-standing lipid membranes (black lipid membranes (BLM)) with enhanced long-term stability have been prepared on these apertures by the methods of Montal and Müller(1,2) as well as Müller and Rudin.(3) The stability and geometric control enables the use of X-ray analysis of free-standing single bilayers. With the presented setup, simultaneous structural and electrophysiological measurements will become feasible. |
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