| STUDIES ON THE MECHANISM OF INDIRUBIN ACTION IN THE TREATMENT OF CHRONIC GRANULOCYTIC LEUKEMIA——V.BINDING BETWEEN INDIRUBIN AND DNA AND IDENTIFICATION OF THE TYPE OF BINDING |
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| 引用本文： | 吴冠芸,刘敬忠,方福德,左瑾.STUDIES ON THE MECHANISM OF INDIRUBIN ACTION IN THE TREATMENT OF CHRONIC GRANULOCYTIC LEUKEMIA——V.BINDING BETWEEN INDIRUBIN AND DNA AND IDENTIFICATION OF THE TYPE OF BINDING[J].中国科学B辑(英文版),1982(10). |
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| 作者姓名： | 吴冠芸 刘敬忠 方福德 左瑾 |
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| 作者单位： | Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing，Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing，Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing，Institute of Basic Medical Sciences，Chinese Academy of Medical Sciences，Beijing |
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| 摘 要： | The binding between indirubin and calf thymus DNA in vitro has been verified by meansof the isotope labelling method, spectrophotometric method and thermal denaturation meas-urements. The λ_max 207 nm of indirubin shifted toward longer wave length with decrease ofabsorbance after the incubation of indirubin with DNA. The escalation of Tm value of DNAinduced by indirubin was about 2.4°C and it was reproducible. The binding force between themwas rather weak, as indirubin molecules were easily released during the precipitation withalcohol or the gel filtration. The binding was not affected by sodium chloride even at high con-centration but greatly decreased (to 20-30% of the control) in the presence of 8 M urea.These results showed that the binding between indirubin and DNA might be of hydrogen bondrather than ionic. The amount of bound ~3H-indirubin was directly proportional to the con-centration of indirubin. However, it increased abruptly when the concentration of indirubinreached 1.5×10~(-4) M. This
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