Imaging and manipulation of adsorbed lipid vesicles by an AFM tip: experiment and Monte Carlo simulations |
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| Authors: | Dimitrievski K Zäch M Zhdanov V P Kasemo B |
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| Affiliation: | 1. Department of Physics, Göteborg University, S-412 96 Göteborg, Sweden;2. Department of Applied Physics, Chalmers University of Technology , S-412 96 Göteborg, Sweden;3. Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia;1. Dipartimento di Fisica, Sapienza Università di Roma, P.zzle A. Moro, 2, 00185 Roma, Italy;2. CNR-IPCF UOS Roma, c/o Dipartimento di Fisica, Sapienza Università di Roma, P.zzle A. Moro, 2, 00185 Roma, Italy;3. Dipartimento di Chimica, Sapienza Università di Roma, P.zzle A. Moro, 5, 00185 Roma, Italy;4. Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Biologia e Biotecnologie “C. Darwin”, Sapienza Università di Roma, P.zzle A. Moro, 5, 00185 Roma, Italy;5. University Institute and Polymer Centre, Faculty of Technology, Tomas Bata University in Zlin, nam. T.G. Masaryka 275, 76272 Zlin, Czech Republic;1. CNR-ISC UOS Roma, c/o Dipartimento di Fisica, Sapienza Università di Roma, P.zzle A. Moro, 2, 00185 Roma, Italy;2. Dipartimento di Fisica, Sapienza Università di Roma, P.zzle A. Moro, 2, 00185 Roma, Italy;3. Laboratoire Charles Coulomb (L2C), UMR 5221 CNRS-Université de Montpellier, Montpellier, France;1. Departamento de Química, Facultad de Ciencias, Universidad de Los Andes, La Hechicera, Mérida 5101, Venezuela;2. Centro de Física Fundamental, Facultad de Ciencias, Universidad de Los Andes, La Hechicera, Mérida 5101,Venezuela;3. Universidad San Francisco de Quito (USFQ), Grupo de Química Computacional y Teórica (QCT-USFQ), Dpto. de Ingeniería Química, 17-1200-841 Quito, Ecuador;1. Department of Chemistry, Jinan University, Guangzhou 510632, PR China;2. Experiment and Technology Center, Jinan University, Guangzhou 510632, PR China;1. Istituto dei Sistemi Complessi (ISC), CNR, sede Sapienza, P.zzle A. Moro, 2, 00185 Roma, Italy;2. Istituto Nazionale per l’Assicurazione contro gli Infortuni sul Lavoro (INAIL), Dipartimento di medicina, epidemiologia, igiene del lavoro e ambientale, Via Fontana Candida 1, 00078 Monte Porzio Catone (RM), Italy;3. Dipartimento di Fisica, Sapienza Università di Roma, P.zzle A. Moro, 2, 00185 Roma, Italy |
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| Abstract: | Single lipid vesicles adsorbed on SiO(2) were manipulated using an atomic force microscope (AFM) operated in contact mode. For large force setpoints, single vesicles were either pushed sideways or ruptured by the tip, depending on the tip type (sharp or blunt) used, while for small force setpoints the vesicles were imaged by the tip. To extend the interpretation of and to guide the experiment, we have developed a generic model of the vesicle-tip-substrate system and performed Monte Carlo simulations, addressing the influence of force setpoint and tip speed and shape on the type of imaging or manipulation observed. Specifically, we have explored AFM-image height and width variations versus force setpoint, typical AFM images for small and large force setpoints, tip-induced vesicle strain versus force setpoint, typical vesicle shapes during pushing for different tip speeds, and the details of vesicle rupture induced by the tip. |
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