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On-line extraction and determination of carbofuran in raw milk by direct HPLC injection on an ISRP column |
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| Authors: | M L Menezes G Felix A C C O Demarchi |
| Institution: | 1. Faculdade de Ciencias, Departmento de Quimica, Universidade Estadual Paulista-UNESP, Av. Edmundo C. Coube S/N, Bauru, CEP-17033-360, Sao Paulo, Brasil 2. Laboratoire de Chimie Organique et Organomtétallique (ENSCPB/CNRS-URA), Av. Pey Berland (PB-108), 33402, Talence, France 3. Laboratorio de Bioquimica, Universidade do Sagrado Corac?o-USC, Rua Irma Arminda, 10-50, Bauru, CEP-17044-160, S?o Paulo, Brasil |
| Abstract: | Summary A method has been developed for extraction and determination of carbofuran in milk. The method involved direct injection of raw milk on to a human serum albumin dimethyloctyl-silica gel (HSA-C8) column and the use of 80:20 (v/v) 0.01 M phosphate buffer pH 5.5-acetonitrile as mobile phase. UV spectrophotometric detection was performed at 220 nm. Identification was based on retention time. Quantification was performed by automatic peak-area determination and was calibrated by use of an external standard. |
| Keywords: | Column liquid chromatography Carbofuran pesticides On-line analysis of milk |
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