银鲳脾脏和肾脏组织细胞体外培养研究

为探究银鲳脾脏和肾脏组织细胞体外培养条件, 本研究采用组织块培养法对银鲳脾脏和肾脏组织进行原代培养. 实验选用DMEM/F-12、M199和DMEM等作为基础培养基, 在其中分别添加体积分数为10％、15％、20％的胎牛血清, 并添加青链霉素及庆大霉素以防细菌污染. 结果表明: 银鲳脾脏和肾脏组织在胎牛血清体积分数为20％的DMEM/F-12培养基中贴壁最好, 迁出细胞的种类最多, 细胞生长速度最快. 其中脾脏组织共迁出6种不同的细胞, 分别为: 免疫细胞、基质细胞、上皮样细胞、成纤维样细胞、造血灶和未知细胞; 肾脏组织共迁出3种细胞, 分别为: 上皮样细胞、巨噬细胞和成纤维样细胞.

The primary culture of cells from spleen and kidney tissues of Pampus argenteus

Tissues from spleen and kidney of the silver pomfret were collected for primary cell culture using tissue piece cultivation method, in order to study the primary cell cultured conditions. DMEM, M199 and DMEM/F-12 supplemented with different concentrations of fetal bovine serum (10％, 15％ and 20％ FBS) and antibiotics (penicillin, gentamicin) were applied to prevent bacterial contamination. The results showed that tissues in DMEM/F-12 with 20％ FBS had the best cell adherence and fastest cell growth, based on cell attachment efficiency and growth rate. The immune cells, stromal cells, epithelioid cells, fibroblast-like cells, and hematopoietic foci were present in the spleen tissue cultures of silver pompano. Hematopoietic foci cells were observed on the stromal layer. In cell cultures of silver pomfret kidney, there were mainly three types of cells. These were the epithelioid cells, macrophages and fibroblast-like cells. The establishment of cell lines from spleen and kidney of silver pomfret would benefit the research on the functions of the fish spleen and kidney.