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Lactate observation in vivo by spectral editing in real time |
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| Authors: | S. Vinitski R. H. Griffey J. Szumowski N. A. Matwiyoff |
| Affiliation: | a Department of Radiology, Thomas Jefferson University Hospital, Philadelphia, PA 19107, USA b Center for Non Invasive Diagnosis, University of New Mexico, Albuquerque, NM, USA c Department of Radiology, University of Rochester, Rochester, NY, USA |
| Abstract: | We have developed a novel in vivo proton MR spectroscopy magnetization transfer method for detection of lactate in ischemic tissue in the presence of interfering fat proton resonances. Pyruvate is magnetically labeled with a saturation pulse and, when converted to lactate, the lactate retains the label. Difference of spectra obtained with and without a saturation pulse contain no fat resonances. High-resolution spectra (determined with a GE 1.5 T Signa) of low lactate levels were obtained in vivo by water suppression using a 2662 composite RF pulse and slice-selective gradients. Spectral subtraction was performed in real time allowing the monitoring of a buildup of the intensity of the lactate peak. Pyruvate-lactate saturation transfer techniques should find wide applicability in the study of ischemia. |
| Keywords: | Author Keywords: NMR Hydrogen spectroscopy Magnetization transfer Metabolites Lactate |
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