A high performance low magnetic field internal electrospray ionization-fourier transform ion cyclotron resonance mass spectrometer |
| |
Authors: | Qinyuan Wu Gordon A Anderson Harold R Udseth Michael G Sherman Steven Van Orden Ruidan Chen Steven A Hofstadler Michael V Gorshkov Dale W Mitchell Alan L Rockwood Richard D Smith |
| |
Institution: | 1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratories, Battelle Boulevard, P8-19, P. O. Box 999, 99352, Richland, WA
|
| |
Abstract: | A new in-magnetic field electrospray ionization (ESI) and Fourier transform ion cyclotron resonance mass spectrometer has been constructed and evaluated. This system is characterized by the use of multiple concentric cryopanels to achieve ultrahigh vacuum in the ion cyclotron resonance cell region, a probe-mounted internal ESI source, and a novel in-field shutter. Initial experiments demonstrate high resolution mass measurement capability at a field strength of 1 T. Mass resolution of 700,000 has been obtained for the 3+ charge state of Met-Lys-bradykinin (at m/z 440) generated by electrospray ionization. When electron impact ionization was employed, resolution in excess of 9,200,000 was achieved for nitrogen molecular ions (N 2 + ). Isotopic resolution for molecular ions of bovine ubiquitin (MW=8565 µ) also was achieved by using small ion populations. |
| |
Keywords: | |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |
|