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An electrochemical investigation of the redox properties of lumiflavin in acidic media
Institution:1. Electroanalysis and Bioelectrochemistry Lab, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, No. 1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan, ROC;2. Sustainable and Smart Materials Research Lab., Department of Nano Science and Technology, Alagappa University, Karaikudi 630 002, Tamil Nadu, India;1. Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology, Shizuoka University, Johoku 3-5-1, Naka-ku, Hamamatsu, Shizuoka 432-8561, Japan;2. Department of Optoelectronics and Nanostructure Science, Graduate School of Science and Technology, Shizuoka University, Johoku 3-5-1, Naka-ku, Hamamatsu, Shizuoka 432-8561, Japan;1. Key Laboratory of Ionic Liquids Metallurgy, Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, Yunnan Province, P.R. China;2. Department of Chemistry, M.M. Engineering College, Maharishi Markandeshwar (Deemed to be University), Mullana, Haryana, India;3. State Key Laboratory of Complex Nonferrous Metal Resources Cleaning Utilization, Kunming University of Science and Technology, Kunming, Yunnan Province, P.R. China;1. Korea Atomic Energy Research Institute, Daejeon, Republic of Korea;2. Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA 16802, USA;3. Institute of Micro and Nanomaterials, Ulm University, 89081 Ulm, Germany;4. Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg 44, Box 2450, B-3001 Heverlee, Belgium
Abstract:The electrochemistry of lumiflavin in acidic media (1% to 40% sulfuric acid) has been investigated using cyclic voltammetry and bulk electrolysis in conjunction with UV—Vis absorption spectroscopy. The observed stabilization of flavin radicals in strongly acidic media has been explained on the basis of differences in medium effects on fully reduced flavin cations (FH+4) as compared with protonated oxidized (FH+2) and radical (FH∓+3) species. The equilibrium constant for the comproportionation reaction between protonated lumiflavin species (FH+2 + FH+4 → 2 FH∓+3) has been determined. Acid dissociation constants for FH+2 and FH+4 have also been evaluated. These data are used in conjunction with the pKa reported for FH∓+3 to estimate the value of the comproportionation constant for the neutral lumiflavin species.
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