N-去甲万古霉素的亲和吸附

通过交联聚丙烯酸甲酯与乙醇胺反应,形成聚(N-羟乙基丙烯酰胺)树脂,在酸催化作用下与环氧氯丙烷反应,形成含有α-羟基氯乙基的树脂.含α-羟基氯乙基的树脂与D-丙氨酸、L-丙氨酸或甘氨酸反应,分别得到含有这3种氨基酸的吸附剂.这3种吸附剂吸附N-去甲万古霉素的结果表明,含D-丙氨酸的吸附剂的吸附量最大,含甘氨酸的吸附剂的吸附量次之,而含L-丙氨酸的吸附剂不吸附N-去万古霉素.说明前两种吸附剂对N-去甲万古霉素存在亲和吸附作用.含D-丙氨酸吸附剂的最佳吸附pH值为5.8,当吸附液中的盐(NaCl)浓度增加时,吸附量降低.用0.4mol/LNa₂CO₃/CH₃CN(摩尔比7∶3,pH=9.5)作为洗脱剂可完全脱附被吸附的N-去甲万古霉素.

Affinity Adsorbents for N-Demethylvancomycin

Poly( N-hydroxyethyl acrylamide) resin was prepared by reacting ethanolamine with crosslinked poly(methyl acrylate). α Hydroxyl chloroethyl groups were introduced into the resin by treating the resin with epichlorohydrin with the acid catalysis. Three adsorbents with D-alanine, Lananine or glycine, respectively, were made by reacting the α hydroxyl chloroethyl group containing resin with the corresponding amino acids. The results of adsorbing N-demethylvan-comycin of the adsorbents indicated that the adsorbent with D-alanine had the highest adsorption capacity, the second was the adsorbent with glycine, while the adsorbent with Lalanine didn't adsorb N-demethylvan-comycin. The optimal pHfor the adsorption for adsorbent with D-alanine was ～5.8 and the adsorption capacity decreased with increasing salt(NaCl) concenttration. The adsorbed N-demethylvan-comycin was completely desorbed with 0.4 mmol/L Na 2CO₃/CH₃CN(molar ratio 7∶3, pH=9.5).