Direct acoustic profiling of DNA hybridisation using HSV type 1 viral sequences |
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| Authors: | Uludağ Yildiz Li Xin Coleman Heather Efstathiou Stacey Cooper Matthew A |
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| Institution: | Akubio Ltd, 181 Cambridge Science Park, Cambridge, UK CB4 0GJ. |
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| Abstract: | We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 x 10(-11) M concentration. |
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