Biosynthesis of a lactate (LA)-based polyester with a 96 mol% LA fraction and its application to stereocomplex formation |
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Authors: | Fumi ShozuiKen&rsquo ichiro Matsumoto,Ren MotohashiJian Sun,Toshifumi SatohToyoji Kakuchi,Seiichi Taguchi |
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Affiliation: | Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo 060-8628, Japan |
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Abstract: | A poly(lactic acid) (PLA)-like terpolyester consisting of 96 mol% lactate (LA), 1 mol% 3-hydroxybutyrate and 3 mol% 3-hydroxyvalerate was produced in recombinant Escherichia coli LS5218 expressing LA-polymerizing enzyme (LPE). The strain was grown on glucose with a feeding of valerate as the monomer precursor. The glass transition and melting temperatures of the terpolyester were close to those of chemically synthesized poly(L-LA)s (PLLAs) having similar molecular weights. Additionally, a blend of the terpolyester, which was composed entirely of (R)-LA (D-LA) due to the strict enantiospecificity of LPE, with PLLA formed a stereocomplex with higher melting temperature (201.9 °C). These results indicate that the biological PLA-like polyester produced via this one-step microbial process has comparable thermal properties to chemically synthesized PLAs. |
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Keywords: | Polylactide Biodegradable plastic Microbial cell factory PDLA Biological PLA |
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