维生素C对生物膜流动性的影响的ESR波谱的研究

本文使用TEMPO和脂肪酸氮氧自由基自旋标记脂质体和正常的与癌变的鼠肝细胞膜,研究了V_c对膜流动性的影响,探讨有关V_c抗肿瘤作用的机理。实验表明:V_c可以影响膜类脂和膜蛋白的运动,提高癌变细胞膜的流动性,并随V_c浓度的增加其作用速度加快,但对正常肝细胞膜流动性则无明显影响。此外,在一定浓度范围内,V_c对正常的与癌变的肝细胞膜的掺入能力有显著差异。

ESRS PECTRA STUDIES OF THE INFLUENCE OF VITAMIN C ON BIOLOGICAL MEMBRANE FLUIDITY

The influence of V_c on membrane fluidity was studied in this artical, by using TEMPO and fatty acid nitroxide spin labeling liposome as -well as normal and malignant cell membrane of mouse liver, in order to explore the anti-cancer mechanism of V_c. The experiments show that V_c can influence the movement of membrane lipids and membrane proteins, increase the fluidity of malignant cell membrane, and the velocity of effect is speeded up with increasing V_c concentration. However, V_c has no evident influence on normal liver cell membrane's fltiidity.In addition, within the studied concen-ration limits,the ability of V_c passing through membrane is obviously different between the normal and malignant cell of mouse liver.