| 同步荧光法研究溴氰菊酯与牛血清白蛋白的结合作用 |
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| 引用本文： | 鹿泽启,王雪荣,贾风燕,殷军港,刘永明. 同步荧光法研究溴氰菊酯与牛血清白蛋白的结合作用[J]. 光谱实验室, 2011, 28(1): 295-298 |
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| 作者姓名： | 鹿泽启 王雪荣 贾风燕 殷军港 刘永明 |
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| 作者单位： | 1. 山东省烟台市农业科学研究院,山东省烟台市福山区南山路26号,265500
2. 烟台大学化学生物理工学院,山东省烟台市清泉路32号,264005 |
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| 基金项目： | 山东省科技攻关项目(NO.2009GG10009053) |
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| 摘 要： | 用同步荧光法消除了溴氰菊酯对牛血清白蛋白内源性荧光的干扰,研究了生理条件（pH=7.4）下溴氰菊酯与牛血清白蛋白之间的相互作用。不同温度下的猝灭常数证明溴氰菊酯对牛血清白蛋白的猝灭是静态过程,据此求得25℃下溴氰菊酯与牛血清白蛋白的结合常数为1.97×105L.mol-1,热力学参数ΔH=29.79kJ.mol-1、ΔS=201.32J.K-1.mol-1,两者之间的相互作用力以疏水作用力为主。根据Foerster非辐射能量转移机理,计算了牛血清白蛋白与溴氰菊酯间结合距离r=5.42nm,能量转移效率E=0.104。
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| 关 键 词： | 溴氰菊酯 牛血清白蛋白 同步荧光 荧光猝灭 |
Study on the Interaction Between Deltamethrin and Bovine Serumim Albumim by Synchronous Fluorescence Spectroscopy |
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| LU Ze-Qi,WANG Xue-Rong,JIA Feng-Yan,YIN Jun-Gang,LIU Yong-Ming. Study on the Interaction Between Deltamethrin and Bovine Serumim Albumim by Synchronous Fluorescence Spectroscopy[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(1): 295-298 |
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| Authors: | LU Ze-Qi WANG Xue-Rong JIA Feng-Yan YIN Jun-Gang LIU Yong-Ming |
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| Affiliation: | LU Ze-Qi WANG Xue-Rong a JIA Feng-Yan a YIN Jun-Gang a LIU Yong-Ming a (Yantai Academy of Agricultral Sciences,Yantai,Shandong 265500,P.R.China)a(School of Chemical and Biological Science and Engineering,Yantai University,Shandong 264005,P.R.China) |
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| Abstract: | The synchronous fluorescence spectrometry was used to eliminate the interference between the emission of the deltamethrin(DM) and the determination of the endogenous fluorescence of the protein,and study the interaction between deltamethrin and bovine serum albumim in physiological condition(pH 7.4).The quenching constant at different temperatures proved that the quenching mechanism of fluorescence of BSA by deltamethrin was a static quenching procedure.The association constant(KB),the enthalpy change and t... |
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| Keywords: | Deltamethrin Bovine Serum Albumim Synchronous Fluorescence Spectrometry Fluorescence Quenching |
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