| 锌离子与牛血清白蛋白相互作用的研究 |
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| 引用本文： | 李凤,荣先国. 锌离子与牛血清白蛋白相互作用的研究[J]. 光谱实验室, 2011, 28(1): 256-258 |
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| 作者姓名： | 李凤 荣先国 |
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| 作者单位： | 滨州医学院烟台校区分析实验室; |
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| 摘 要： | 用荧光光谱法研究了锌离子(Zn2+)与牛血清白蛋白(BSA)的作用机制.实验表明,Zn2+对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭.求出了猝灭常数、结合常数及结合位点数.利用同步荧光光谱研究了Zn2+对BSA分子荧光光谱的影响.
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| 关 键 词： | Zn2+ 牛血清白蛋白 荧光光谱 静态猝灭 |
Interaction Between Zinc Ion and Bovine Serum Albumin |
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| LI Feng,RONG Xian-GuO. Interaction Between Zinc Ion and Bovine Serum Albumin[J]. Chinese Journal of Spectroscopy Laboratory, 2011, 28(1): 256-258 |
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| Authors: | LI Feng RONG Xian-GuO |
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| Affiliation: | LI Feng RONG Xian-Guo(Chemical Analysis Laboratory,Binzhou Medical College,Yantai,Shandong 264003,P.R.China) |
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| Abstract: | The interaction between zinc ion(Zn2+) and bovine serum albumin(BSA) was studied by fluorescence spectrometry.The fluorescence of BSA was quenched by Zn2+,and the combination of which was a static quenching process.The quenching data were analyzed according to Stern-Volmer equation,the quenching constant,binding constant and binding sites were obtained.The influence of Zn2+ to fluorescence spectrum of BSA was investigated by synchronous fluorescence spectroscopy. |
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| Keywords: | Zinc Ion Bovine Serum Albumin Fluorescence Spectrum Static Quenching |
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