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Fabrication of monolithic frits and columns for chip-based multidimensional separation devices |
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| Authors: | Noor Abdulhussain Suhas H Nawada Sinéad A Currivan Peter J Schoenmakers |
| Institution: | 1. Van ’t Hoff Institute for Molecular Science (HIMS), University of Amsterdam, Amsterdam, The Netherlands;2. Van ’t Hoff Institute for Molecular Science (HIMS), University of Amsterdam, Amsterdam, The Netherlands
Centre for Analytical Sciences Amsterdam (CASA), University of Amsterdam, Amsterdam, The Netherlands;3. Department of Applied Science, Technological University Dublin, Tallaght, Ireland MiCRA Biodiagnostics, Technological University Dublin, Tallaght, Ireland Centre of Applied Science for Health (CASH), Technological University Dublin, Tallaght, Ireland |
| Abstract: | In this work, devices for two-dimensional separations are considered. The device contains a flow distributor, a first-dimension channel, and 17 second-dimension outlets. In the design, all connections between the first-dimension channel, the flow distributor, and the second-dimension outlets were tapered, with a minimal diameter of 20 μm. The use of photo-masking is explored for the fabrication of monolithic frits in all tapered connections. Monolithic frits with optimized permeability and length were successfully fabricated in all 33 tapered channels through light-induced polymerization, photo-masking, and selective exposure. The efficacy of the monolithic frits was demonstrated by creating a packed bed of 15-μm particles, confined within the first-dimension channel. The outlet of the first-dimension channel was successfully connected to a mass spectrometer. Effective flow confinement was demonstrated with a reversed-phase separation of a mixture of five standard peptides. |
| Keywords: | first-dimension separation glass chip microfluidics multidimensional chromatography spatial separations |