Optimization of the extraction process of flavonoids from Trollius ledebouri with natural deep eutectic solvents |
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Authors: | Jiale Zuo Peirong Ma Shuqin Geng Yangzhi Kong Xiang Li Zhaosheng Fan Yanling Zhang Alideertu Dong Qun Zhou |
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Institution: | 1. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, P. R. China
Engineering Research Center of Dairy Products Quality and Safety Control Technology, Ministry of Education, Inner Mongolian University, Hohhot, P. R. China;2. Technology Center, Shanghai Tobacco Group Beijing Cigarette Factory Co., Ltd., Beijing, P. R. China;3. College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, P. R. China;4. Department of Chemistry, Tsinghua University, Beijing, P. R. China |
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Abstract: | In recent years, natural deep eutectic solvents have been favored greatly due to their environment friendly, mild biological toxicity and simple biodegradability. Natural deep eutectic solvents gradually applied for the extracting bioactive compounds from natural products efficiently. In this study, 20 natural deep eutectic solvents were prepared and their physical and chemical properties were tested. The ultrasonic-assisted extraction method was used to extract flavonoids from Trollius ledebouri and high-performance liquid chromatography-ultraviolet was applied to examine two main bioactive flavonoids (orientin and vitexin). Compared with traditional solvents (water and 60% ethanol solution), natural deep eutectic solvents composed of L(-)-proline and levulinic acid (molar ratio 1:2) show a super extraction efficiency. On this basis, the response surface method was used to optimize the extraction temperature, extraction time, water contents, and solid–liquid ratio. As a consequence, the extraction temperature 60℃, extraction time 18 min, water content 14% (v/v), and the solid–liquid ratio 48 mL·g−1 were chosen as the best extraction process. This study shows that natural deep eutectic solvents can effectively extract flavonoids from T. ledebouri, laying a foundation for the further application of natural deep eutectic solvents to extract bioactive compounds from natural products. |
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Keywords: | flavonoids natural deep eutectic solvents response surface method Trollius ledebouri |
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