Rapid resolution liquid chromatography (RRLC) analysis for quality control of Rhodiola rosea roots and commercial standardized products |
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Authors: | Ma Yuan-Chun Wang Xiao-Qiang Hou Fei Fei Ma Jie Luo Mai Lu Shane Jin Peter Terevsky Nelly Chen Alice Xu Iris Pate Asmita V Gorecki Dariusz |
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Affiliation: | Canadian Phytopharmaceuticals Corp., 12233 Riverside Way, Richmond, B.C. Canada V6W 1K8. |
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Abstract: | A simple, sensitive and reliable reversed phase Rapid Resolution Liquid Chromatography (RRLC) method was developed and validated for six biologically active compounds (salidroside, tyrosol, rosarin, rosavin, rosin and rosiridin) in Rhodiola rosea L. roots and powder extracts. The method uses a Phenomenex C18 (2)-HST column at 40 degrees C with a neutral gradient system mobile phase (H20 and acetonitrile), a flow rate of 1.0 mL/min, and UV detection wavelengths set at 205 and 254 nm, simultaneously. Baseline separation of the six active compounds was achieved within 8 minutes. The average percentages of rosavins (rosarin, rosavin, and rosin) in authentic R. rosea roots and root powder extracts were quantitatively determined and a characteristic R. rosea roots RRLC profile was established. The RRLC method is accurate and sensitive; in addition, it effectively increases the sample analysis throughput compared with conventional HPLC. |
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