Characterizing 3-chloro-p-toluidine hydrochloride on rough-hulled rice and ethyl-cellulose-coated rice baits using high-performance liquid chromatography

Methods are developed to extract and quantitate the avicide 3-chloro-p-toluidine hydrochloride (CPT HCl) from rough-hulled rice and ethyl-cellulose-coated rice baits using high-performance liquid chromatography. The mobile phase used in the ethyl-cellulose-coated rice matrix method is an acetonitrile(ACN)-phosphate buffer (60:40) at pH 8, and the rough-hulled rice matrix method uses an CAN-phosphate (70:30) buffer at pH 2. Increased retention time is observed for CPT HCl at the higher pH. The two methods have been useful in characterizing different bait formulations in an ongoing pesticide formulation improvement program.