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高效液相色谱法测定尿液中的异硫氰酸酯
引用本文:褚兴棣,朱莉芳,高玉堂.高效液相色谱法测定尿液中的异硫氰酸酯[J].色谱,2004,22(1):30-32.
作者姓名:褚兴棣  朱莉芳  高玉堂
作者单位:上海市肿瘤研究所流行病学研究室,上海,200032
摘    要: 省去合成1,3 苯二硫杂环五烯 2 硫酮这一步骤,直接用异硫氰酸丙基酯与1,2 苯二硫酚反应作标准,建立了尿液中异硫氰酸酯的反相高效液相色谱(HPLC)测定方法。异硫氰酸丙基酯的标准曲线回归方程 y =0.418 2x + 2.821 ( r2 = 0.999 3 )与异硫氰酸甲基酯的回归方程 y = 0.412 2x + 2.442 3 ( r 2= 0.996 6 )基本拟合。检出限(以信噪比为2.5计)为0.08 μ mol/L 。日内重现性( n =21)以相对标准偏差(RSD)表

关 键 词:高效液相色谱  异硫氰酸酯  尿液  异硫氰酸甲基酯  异硫氰酸丙基酯
文章编号:1000-8713(2004)01-0030-03
修稿时间:2003年3月18日

Determination of Isothiocyanates in Human Urine by High Performance Liquid Chromatography
CHU Xingdi,ZHU Lifang,GAO Yutang.Determination of Isothiocyanates in Human Urine by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2004,22(1):30-32.
Authors:CHU Xingdi  ZHU Lifang  GAO Yutang
Institution:Department of Epidemiology, Shanghai Cancer Institute, Shanghai 200032, China. xdchu@sci.shmu.edu.cn
Abstract:A reversed-phase high performance liquid chromatographic method (HPLC) was established for determination of isothiocyanates in human urine, which used the reaction of propyl isothiocyanate with 1,2-benzenedithiol as reference instead of the synthesis of 1,3-benzodithiole-2-thione. The linear regression of the standard curve was determined to be y = 0.418 2 x + 2.821 (r2 = 0.999 3), that was well matched with the regression of methyl isothiocyanate, y = 0.412 2x + 2.442 3(r2 = 0.996 6). The detection limit (at approximately S/N = 2.5) was 0.08 micromol/L. The mean relative standard deviation (RSD) (n = 21) for the intra-day reproducibility was (4.7 +/- 2.9)%. The mean RSD (n = 4) for inter-day reproducibility of three samples was (7.0 +/- 5.0)%. The recovery of four propyl isothiocyanate standards was (91.3 +/- 7.9)% (mean RSD 8.7%). The initial results obtained by this method may provide useful information regarding to the kinetics of isothiocyanates in dietary consumption and excretion of individuals.
Keywords:high performance liquid chromatography  isothiocyanates  human urine  methyl isothiocyanate  propyl isothiocyanate  
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