甲状腺癌转移性淋巴结的红外光谱研究

联合运用衰减全反射(attenuated total reflectance，ATR)探头与FTIR光谱仪，测定了新鲜离体的甲状腺癌转移性颈部淋巴结20枚和非转移性淋巴结69枚的FTIR光谱，比较两组光谱13个谱带的峰位及相对峰强等28个指标，找出甲状腺癌转移性淋巴结光谱的特征。与非转移性淋巴结相比，转移性淋巴结光谱中与蛋白、脂质、糖类、核酸相关的谱带均发生了明显的变化，具体如下：(1)与蛋白质相关的谱带的相对峰强I_{3 280}/I1 460(P=0.029)，I_{1 640}/I_{1 460}(P=0.019)，I_{1 546}/I_{1 460}(P=0.004)均显著升高;(2)脂类相关的相对峰强I_{1 743}/I_{1 460}显著降低(P=0.026)，而I_{1 400}/I_{14 60}则明显增高(P=0.001);(3)与糖类相关的1 165与1 120 cm-1处峰位P_{1 165}(P=0.019)，P_{1 120}(P=0.000)发生明显蓝移(向高波数移动)，相对峰强I1 165/I_{1 460}显著降低(P=0.006);(4)与核酸相关的1 085 cm-1处峰位P_{1 085}发生明显地红移(向低波数移动)(P=0.029);(5)其他指认未明的谱带的相对峰强I_{1 303}/I_{1 460}(P=0.008)，I_{1 303}/I1 240(P=0.039)均明显升高。与非转移性淋巴结光谱相比较，甲癌转移的淋巴结光谱在多个谱带的峰位或相对峰强方面存在显著的差异。FTIR可应用于术中甲状腺癌转移性淋巴结的探测。

Investigation on Fourier Transform Infrared Spectrum of Metastatic Lymph Node in Thyroid Cancer

The aim of the study is to explore the possibility of detecting metastatic lymph nodes by FTIR spectra during the surgery of thyroid cancer. The FTIR spectra of 20 metastatic lymph nodes and 69 non-metastatic ones were collected via an ATR (attenuated total reflectance) probe. For each spectrum,28 variables of 13 bands including peak positions and relative intensities were measured. The variables of metastatic lymph node were compared to those of non-metastatic ones using standard statistic methods. The res...