Subtiligase as a hydrothiolase for the synthesis of peptide thioacids |
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Authors: | Xiao-Hong Tan |
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Institution: | Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore |
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Abstract: | Subtiligase catalyzes the hydrolysis or the aminolysis of a peptide glycolate ester substrate via an acyl-enzyme thioester intermediate. We show that this intermediate can be intercepted by a hydrosulfide ion to generate a peptide thioacid as the hydrothiolysis product. Also shown is the use of the so-prepared peptide thioacids in mini thiol capture ligation. |
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