Characterization of dextrans by size-exclusion chromatography on unmodified silica gel columns, with light-scattering detection, and capillary electrophoresis with laser-induced fluorescence detection |
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Authors: | Josef Chmelí k Jitka Chmelí kov Milos V. Novotny |
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Affiliation: | Department of Chemistry, Indiana University, Bloomington, IN 47405, USA |
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Abstract: | Unmodified silica gel size-exclusion columns were used in an on-line combination with light-scattering detection for a size characterization of dextrans. The influence of electrostatic interactions on analyte retention was briefly investigated. Size-exclusion chromatography was also used for evaluation of the fluorescence labeling procedure for dextrans with 8-aminonaphthalene-1,3,6-trisulfonic acid. The derivatives obtained through this procedure were used for electrophoretic measurements with laser-induced fluorescence detection. A comparison between the size-exclusion data and capillary electrophoresis indicates that the effectiveness of fluorescent labeling decreases with molecular mass of the dextran analytes. |
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Keywords: | Dextrans Polymers Polysaccharides Aminonaphthalene trisulfonate |
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