A miniature laser speckle fluorescence sectioning microscope for cell imaging |
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| Authors: | Yonghong Shao Heng Li Qiao Wen Yan Wang Junle Qu Hanben Niu Key Laboratory of Optoelectronic Devices Systems of Ministry of Education Guangdong Province |
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| Affiliation: | Yonghong Shao,Heng Li,Qiao Wen,Yan Wang,Junle Qu,and Hanben Niu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Institute of Optoelectronics,Shenzhen University,Shenzhen 518060,China |
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| Abstract: | We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state (DPSS) laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. Optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. High contrast fluorescence sectioning images of bovine pulmonary ar... |
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