Spectral imaging of time-resolved anisotropy: theory and experiment |
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Authors: | Yanzhou Zhou Qingruo Wang Jingsong He Lerong Lin Faculty of Automation Guangdong University of Technology Guangzhou China |
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Affiliation: | Yanzhou Zhou,Qingruo Wang,Jingsong He,and Lerong Lin Faculty of Automation,Guangdong University of Technology,Guangzhou 510006,China |
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Abstract: | Time-resolved fluorescence anisotropy on the nanosecond time scale is useful for the study of the rapid rotation of macromolecules. A system combining the capabilities of fluorescence spectral imaging with time-resolved fluorescence anisotropy and enabling the wide-field measurement of the spectroscopic parameters of fluorophores is discussed. The phasor approach is used to quantitatively analyze the time-resolved fluorescence anisotropy by transforming the polarized parallel and perpendicular components to... |
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