Fluorescence lifetime imaging of molecular rotors to map microviscosity in cells |
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| Authors: | James A. Levitt Marina K. Kuimova Gokhan Yahioglu Pei-Hua Chung Klaus Suhling David Phillips |
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| Affiliation: | [1]Department of Physics, King＇s College London, Strand, London WC2R 2LS, UK [2]Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ, UK [3]PhotoBiotics Ltd., 21 Wilson Street, London EC2M 2TD, UK |
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| Abstract: | Fluorescence liftime imaging (FLIM) of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their environment. Incubating cells with these dyes, we find a punctate and continuous distribution of the dye in cells. The viscosity value obtained in what appears to be endocytotic vesicles in living cells is around 100 times higher than that of water and of cellular cytoplasm.Time-resolved fluorescence... |
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