Improving the precision of fluorescence lifetime measurement using a streak camera |
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| Authors: | Heng Li Yonghong Shao Yan Wang Junle Qu Hanben Niu Key Laboratory of Optoelectronic Devices Systems of Ministry of Education Guangdong Province |
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| Affiliation: | Heng Li,Yonghong Shao,Yan Wang,Junle Qu,and Hanben Niu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,Institute of Optoelectronics,Shenzhen University,Shenzhen 518060,China |
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| Abstract: | Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. We have constructed a two-photon excitation fluorescence lifetime measurement system that is based on a synchroscan streak ca... |
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