PCB-based integration of electrochemiluminescence detection for microfluidic systems |
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Authors: | Pittet Patrick Lu Guo-Neng Galvan Jean-Marc Ferrigno Rosaria Blum Loïc J Leca-Bouvier Béatrice |
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Affiliation: | Institut des Nanotechnologies de Lyon (INL), CNRS UMR5270, Université Claude Bernard Lyon 1, 43 boulevard du 11 novembre 1918, F-69622 Villeurbanne, France. Patrick.Pittet@univ-lyon1.fr |
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Abstract: | This communication presents an instrumental development based on the printed circuit board (PCB) technology to integrate electrochemiluminescence (ECL) analysis in microfluidic systems. PCB gold macro- (10 mm2) and micro- (0.09 mm2) electrodes and two ECL microfluidic devices are designed, fabricated and tested via luminol ECL detection. Potential modulation is performed between 0.7 and 0 V vs. Ag/AgCl for luminol oxidation, thus giving rise to on/off ECL responses in the presence of hydrogen peroxide. Synchronous detection is adopted to allow weak ECL signal recovery at a very low signal-to-noise ratio (SNR). The detection limit obtained with the two ECL microfluidic devices is 50 nM and 100 nM H2O2 for macroelectrodes and microelectrodes, respectively. |
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