A simple and selective kinetic spectrophotometric method for the determination of kanamycin using acetylacetone-formaldehyde reagent in N,N′-dimethylformamide medium |
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| Authors: | A S Ahmad MD N Hoda M Ahmad F Islam S Z Qureshi |
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| Institution: | (1) Analytical Research Laboratory, Department of Chemistry, Aligarh Muslim University, Aligarh, 202002 (UP), India;(2) Department of Anesthesiology and Critical Care Medicine Baltimore, The Johns Hopkins University School of Medicine, MD 21205, USA;(3) Neurotoxicology Lab., Dept. of Toxicology, Hamdard University, New Delhi, 110062, India |
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| Abstract: | A simple, sensitive, and selective kinetic spectrophotometric method for the determination of kanamycin in pure form and pharmaceutical
formulations is described. The method is based on the measurement of the intensity of the yellow chromogen formed by the reaction
between kanamycin and acetylacetone-formaldehyde reagent in a N,N′-dimethyl formamide medium. The variable-time method was used to evaluate the rate of reaction of the colored chromogen formed
at 410 nm. The reaction conditions were optimized and the calibration graph was found to be linear in the range 60–160 μg/mL.
The results obtained by the developed and reference methods are in good agreement. Various statistical parameters were evaluated
to establish the precision, accuracy, repeatability, and reproducibility of the proposed method.
The text was submitted by the authors in English. |
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