Direct modification of mRNA by ferrocenyl carbodiimide and its application to electrochemical detection of mRNA. |
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| Authors: | Kosuke Mukumoto Takahiko Nojima Shinobu Sato Michinori Waki Shigeori Takenaka |
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| Institution: | Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan. |
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| Abstract: | Ferrocenyl carbodiimide (1) could be used for the direct labeling of synthetic RNA and expressed mRNA in vitro with the electrochemically active ferrocene moieties. These RNAs modified by 1 could be detected electrochemically coupled with a DNA probe-immobilized electrode. After hybridization of 1.1 Kb mRNA modified by 1 with the DNA probe-immobilized electrode, the peak charge observed by an Osteryoung square wave voltammetry (SWV) measurement correlated well with the concentration of mRNA, having a detection limit at the sub nanogram level. |
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