| 表面等离子体共振技术用于蛋白对氨基酸手性识别的动力学研究 |
| |
| 引用本文： | 申刚义,高妍,戴东升,崔箭,刘裕明,裴凌鹏. 表面等离子体共振技术用于蛋白对氨基酸手性识别的动力学研究[J]. 高等学校化学学报, 2011, 32(8): 1709-1713 |
| |
| 作者姓名： | 申刚义 高妍 戴东升 崔箭 刘裕明 裴凌鹏 |
| |
| 作者单位： | 1. 中央民族大学中国少数民族传统医学研究院, 中国少数民族传统医学国家民委-教育部重点实验室, 北京 100081; 2. 亚宝药业集团股份有限公司, 太原 044602 |
| |
| 基金项目： | 国家自然科学基金(批准号:81001595); 中央高校基本科研业务费专项资金中央民族大学自主科研项目(批准号:0910KYQN67); 新疆名医名方与特色方剂学实验室开放课题(批准号:XJDX0910-2010-01)资助 |
| |
| 摘 要： | 利用SPR技术，以牛血清白蛋白和溶菌酶为探针构筑手性识别传感膜，开展了对L-和D-苯丙氨酸以及L-和D-色氨酸手性识别的动力学研究。实验结果表明，两种蛋白在与每种氨基酸分子的L-和D-型异构体相互作用过程都存在明显的动力学差异。动力学数据进一步显示两种蛋白与每种氨基酸L-型异构体的亲和力均大于D-型。
|
| 关 键 词： | 表面等离子体共振 手性识别 氨基酸 动力学 |
| 收稿时间： | 2011-01-21 |
Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance |
| |
| SHEN Gang-Yi,GAO Yan,DAI Dong-Sheng,CUI Jian,LIU Yu-Ming,PEI Ling-Peng. Kinetics Study of Chiral Recognition Between Protein and Amino Acid Enantiomers by Surface Plasmon Resonance[J]. Chemical Research In Chinese Universities, 2011, 32(8): 1709-1713 |
| |
| Authors: | SHEN Gang-Yi GAO Yan DAI Dong-Sheng CUI Jian LIU Yu-Ming PEI Ling-Peng |
| |
| Affiliation: | 1. Key Laboratory of Chinese Minority Traditional Medicine, State Ethnic Affairs Commission and Ministry of Education of China, Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;2. Yabao Pharmaceutical Group Co. Ltd., Taiyuan 044602, China |
| |
| Abstract: | Based on surface plasmon resonance(SPR) technique,a new method was developed for efficiently recognizing the chiral discrimination between the small molecule L-and D-amino acid enantiomers without any labeling.Bovine serum albumin and lysozyme were selected as chiral recognition probes,and phenylalanine and tryptophan including four L-,D-enantiomers were used as analytes.While six different concentrations of each amino acid enantiomers interacted with two protein probes at the same time,the binding kinetics... |
| |
| Keywords: | Suface plasmon resonance(SPR) Chiral recognition Amino acid Kinetics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学学报》浏览原始摘要信息 |
|
点击此处可从《高等学校化学学报》下载全文 |
|
