检测活性氧的荧光探针新进展

活性氧对于人体是十分重要的。然而,过量的活性氧是相当有害的,它们会对人体产生氧化损伤,导致细胞死亡。活性氧现在已经引起了化学、生物、医药等多个领域学者的浓厚兴趣,它们被认为和多种病理条件有密切的联系。由于活性氧寿命短、反应活性高,并且大部分都存在于体内很难被捕获,因此它们的分析测定是一项国际性难题。荧光探针作为活性氧的高灵敏的检测分析物,已经得到越来越广泛和深入的研究。由于每一种活性氧都有它独特的生理学活性,因此设计高选择性的,能够检测具体一种活性氧的荧光探针分子就显得十分重要。本文主要对近三年来检测单线态氧、过氧化氢、超氧阴离子和羟自由基这四种活性氧的荧光探针的研究进展进行综述,关注这类荧光探针的检测机理以及具体应用。

Recent Advances in Fluorescent Probes for the Detection of Reactive Oxygen Species

Reactive oxygen species（ROS） are essential to life.However,when these reactive species were overproduced,it become highly harmful,causing oxidative stress and leading cell death.ROS have captured the interest of many researchers in the chemical,biological,and medical fields since they are thought to be associated with various pathological conditions.ROS present some characteristics that make them difficult to detect,namely their very short lifetime,high reactive activity and incapable capturing due to existing in vivo. Fluorescent probes are excellent sensors of ROS due to their high sensitivity,have become the research focuses. It is important to design high selective and specific detecting fluorescent probe because each ROS has its own unique physiological activily. This review focused on the detection mechanism and applications of fluorescent probes for the detection of singlet oxygen （^1O2）, hydrogen peroxide （H2O2）, superoxide anion （O2^-·）, or hydroxyl radicals （·OH）,a field in which remarkable progress had been achieved in the last three years.