| 根癌农杆菌对黄瓜和朱顶红的转化 |
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| 引用本文： | 梁玉玲,朱宝成,王俊刚,李庆余.根癌农杆菌对黄瓜和朱顶红的转化[J].河北大学学报(自然科学版),1994(3). |
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| 作者姓名： | 梁玉玲 朱宝成 王俊刚 李庆余 |
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| 作者单位： | 保定师专生物系 |
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| 摘 要： | 用携带有构建质粒Ｃｏ１８的根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）菌株ＧＶ３１１－ＳＥ转化双子叶植物黄瓜子叶和单子叶植物朱顶红鳞叶，得到了抗卡那霉素的黄瓜植株和朱顶红愈伤组织，并测出了ＮｐＤＨ活性，证明构建质粒Ｃｏ１８所携带的外源基因转移进了这两种植物细胞并得到了表达。
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| 关 键 词： | 根癌农杆菌，转化 |
In Vitro Transformation of Cucumber and Barbadoslily by Agrobacterium tume faciens |
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| Liang Yuling Zhu Baocheng Wang Jungang LiQingyu.In Vitro Transformation of Cucumber and Barbadoslily by Agrobacterium tume faciens[J].Journal of Hebei University (Natural Science Edition),1994(3). |
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| Authors: | Liang Yuling Zhu Baocheng Wang Jungang LiQingyu |
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| Abstract: | Dicotyls cucumber and monocot barbadoslily were transferred byreconstructed Ti-plasmid Of Agrobacterium tume faciens.The regenerated plantlets of cu-cumber and calli of barbadoslily were obtained on the medium containing 100mg/Lkanamycin,and the NpDH activity was detected.This suggested the foreign gene harhoredin reconstructed plasmid Co-18 were integrated into the above plants and expressed. |
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| Keywords: | Agrobacterium tume faciens Transformation |
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