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Mixer-settler counter-current chromatography with a barricaded spiral disk assembly with glass beads |
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| Authors: | Ito Yoichiro Qi Lin Powell Jimmie Sharpnack Frank Metger Howard Yost James Cao Xue-Li Dong Yin-Mao Huo Liang-Sheng Zhu Xiao-Ping Li Ting |
| Affiliation: | Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-8014, USA. itoy2@mail.nih.gov |
| Abstract: | A novel spiral disk is designed by placing barricades at 6 mm intervals in the middle of the spiral channel to divide the channel into multiple sections. Glass beads are placed in every other section so that the planetary motion produces repetitive mixing and settling of polymer phase systems. Performance of this mixer-settler spiral disk assembly was examined for separation of lysozyme and myoglobin with a polymer phase system. The best results were obtained with a spiral disk equipped with barricades with openings ranging from 1.2 to 0.4 mm on each side at a high revolution speed up to 1200 rpm. |
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