Three-dimensional holographic reconstruction of two-dimensional image information from serial sections and its applications in biomedicine |
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| Authors: | Guo-Ping Zhou |
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| Institution: | The author is in the Biomedical Engineering section, Department of Precision Instruments, Shanghai Jiaotong University, Shanghai 200030, People''s Republic of China |
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| Abstract: | An experimental study to synthesize holographically two-dimensional projections of serial sections at various depths into three-dimensional images is described. The effects caused by the position, size and resolution of virtual images during reconstruction are analysed, and the advantages and limitations of this method are discussed. An application of this experimental method — the three-dimensional reconstruction of serial sections of a newt embryo — is presented. |
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| Keywords: | holography multiple image reconstruction |
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