The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection. |
| |
Authors: | Yoshio Suzuki Hidetoshi Arakawa Masako Maeda |
| |
Affiliation: | School of Pharmaceutical Sciences, Showa University, Shinagawa, Tokyo 142-8555, Japan. |
| |
Abstract: | Immunoassay is widely employed as a highly sensitive, specific analytical method for hormones and drugs in biological samples. A technique utilizing capillary electrophoresis with laser-induced fluorescence detection was examined based on the reaction process of these immunoassays in order to develop a protocol characterized by high sensitivity and high speed. The conditions of the antigen-antibody reaction and capillary electrophoresis were variously examined using fluorescein-labeled methotrexate and the antibody of methotrexate. As a result, the immunoassay could be completed within a few minutes. Moreover, detection in the pg range could be accomplished. The sensitivity corresponded to that of radioimmunoassay. A simultaneous multi-component analysis of the immunoassay is also possible due to the high resolving power of capillary electrophoresis. In this study, the possibility of a simultaneous analysis of methotrexate and vancomycin was also investigated. |
| |
Keywords: | |
|
|