基于单胺类神经递质代谢谱研究姜黄和郁金的寒热药性差异

为了研究姜黄、郁金寒热药性差异，采用HPLC-MS建立了大鼠脑中7种儿茶酚胺类物质的分析方法，对寒、热证候模型，及姜黄、郁金干预后相应模型中大鼠脑中儿茶酚胺类代谢物进行了分析。试验数据采用主成分分析（PCA）进行处理,采用t检验判别主要差异代谢产物。结果表明：大鼠脑中儿茶酚胺类成分与寒、热因素存在相关性，主成分得分图（PCA Scatter Plot）可以清楚的把寒、热模型和正常对照组进行区分，主要差异代谢物为E,3-MT，5-HT；姜黄对寒证模型的作用较为明显，模式识别可以区分二者，主要差异代谢物为E、3-MT，说明了姜黄具有温性药性的性质；郁金、姜黄对热证模型均有一定作用，但郁金的作用强于姜黄,主要差异代谢物为E、3-MT、5-HT、DOPAC。

Heat and Cold Properties of Jianghuang and Yujin Based on Brain Catecholamine Metabolic Profiling

To compare and confirm different effects of Jianghuang and Yujin,a HPLC-MS method for the quantitative determination of 7 catecholamines in brain samples was developed.Principal component analysis(PCA) was processed to analyze the catecholamine metabolic profiling.The results suggested that heat-syndrome model and cold-syndrome model could be distingushed by catecholamine metabolic profiling.Jianghuang and Yujin have different effects for the heat and cold syndrome model based on brain catecholamines metabo...