| 基于分子印迹技术的牛血清白蛋白电位式传感器的研究 |
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| 引用本文： | 常子栋,高楠莎,李一峻,何锡文. 基于分子印迹技术的牛血清白蛋白电位式传感器的研究[J]. 分析试验室, 2012, 0(4): 20-22 |
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| 作者姓名： | 常子栋 高楠莎 李一峻 何锡文 |
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| 作者单位： | 南开大学化学学院 |
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| 基金项目： | 国家自然科学基金项目(21075067);湖南大学化学生物传感与计量学国家重点实验室项目(2007003)资助 |
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| 摘 要： | 以牛血清白蛋白(BSA)为模板,合成了分子印迹聚合物凝胶(MIP),进而制得了以聚氯乙烯(PVC)为支撑膜的BSA电位式传感器。采用直接电位法测得BSA在浓度0.1～1.0 mg/mL范围内与电位呈线性关系。此电极制作简单,可用于BSA的测定。
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| 关 键 词： | 牛血清白蛋白 分子印迹技术 电位式传感器 |
Preparation of a potentiometric sensor for bovine serum albumin based on molecular imprinting technology |
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| CHANG Zi-dong,GAO Nan-sha,LI Yi-jun and HE Xi-wen. Preparation of a potentiometric sensor for bovine serum albumin based on molecular imprinting technology[J]. Chinese Journal of Analysis Laboratory, 2012, 0(4): 20-22 |
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| Authors: | CHANG Zi-dong GAO Nan-sha LI Yi-jun HE Xi-wen |
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| Affiliation: | (College of Chemistry,Nankai University,Tianjin 300071) |
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| Abstract: | A molecular imprinted polymer gel(MIP) was synthesized using bovine serum albumin(BSA) as the template molecule.A MIP membrane electrode was prepared with polyvinyl chloride(PVC) as the supporting membrane.The results indicated that the sensor exhibited good potential response to BSA,and the potential of BSA was linear over the logarithm of concentration in the range of 0.1~1.0 mg/mL.The sensor had no response for lysozyme(Lys) and the smaller response of bovine hemoglobin(BHB) compared with the same concentration of BSA.The MIP membrane electrode can be applied to the determination of BSA. |
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| Keywords: | Bovine serum albumin Molecular imprinting technology Potentiometric sensor |
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