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| 人肝细胞中总砷及砷代谢产物分析方法研究 | |
| 引用本文： | 曹煊,余晶晶,王庚,于振花,杨黄浩,徐苑苑,孙贵范,王小如.人肝细胞中总砷及砷代谢产物分析方法研究[J].分析化学,2009,37(1). |
| 作者姓名： | 曹煊 余晶晶 王庚 于振花 杨黄浩 徐苑苑 孙贵范 王小如 |
| 作者单位： | 1. 中国海洋大学化学化工学院,青岛,266003;国家海洋局第一海洋研究所,青岛,266061 2. 国家海洋局第一海洋研究所,青岛,266061 3. 山东大学化学与化工学院,济南,250100 4. 国家海洋局第一海洋研究所,青岛,266061;中国科学院海洋研究所,青岛,266071 5. 中国医科大学公共卫生学院,沈阳,110001 |
| 摘 要： | 本工作研究了冷消解、微波消解、高压密闭消解方法对超声破碎后细胞中砷的ICP-MS测定的影响,并与直接进样测定结果作对比.结果表明,微波消解法和高压密闭消解法效果较好,测定的精密度以RSD表示,分别为2.1%和1.2%;日间6次测定的精密度分别为1.2%和2.0%;元素加标回收率均在95.7%～108.1%范围.用4种消解方法对应的ICP-MS方法检出限介于0.74～0.93 μg/l之间,符合痕量分析要求.本工作还建立了高效液相色谱(HPLC)与电感耦合等离子体质谱(ICP-MS)联用技术对1 μmol/L染砷组细胞样品中的砷化学形态进行了初步探讨,发现细胞内除了存在无机砷(As(Ⅲ)和As(Ⅴ))外,同时存在二甲基砷(DMA)和一种甲基砷中间代谢产物. |
| 关 键 词： | 细胞消解 高效液相色谱 电感耦合等离子体质谱 肝细胞 砷化学形态 |
Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes |
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| CAO Xuan,YU Jing-Jing,WANG Geng,YU Zhen-Hua,YANG Huang-Hao,XU Yuan-Yuan,SUN Gui-Fan,WANG Xiao-Ru.Determination of Total Arsenic and Arsenic Metabolites in Human Liver Hepatocytes[J].Chinese Journal of Analytical Chemistry,2009,37(1). | |
| Authors: | CAO Xuan YU Jing-Jing WANG Geng YU Zhen-Hua YANG Huang-Hao XU Yuan-Yuan SUN Gui-Fan WANG Xiao-Ru |
| Institution: | College of Chemistry and Chemical Engineering;Ocean University of China;Qingdao 266003;First Institute Oceanography of State Oceanic Administration;Qingdao 266061;College of Chemistry and Chemical Engineering;Shandong University;Jinan 250100;Institute of Oceanology Chinese Academy of Science;Qingdao 266071;College of Pubic Health;China Medical University;Shenyang 110001 |
| Abstract: | The effects of direct sampling and three digestion methods were investigated on the determination of arsenic in Chang liver hepatocytes after ultrasonic disintegration were investigated.The results showed that the efficiency of microwave digestion and obturator digestion was better than cold digestion and direct sampling.The day precision(present as RSD) of microwave digestion and obturator digestion were 2.1% and 1.2%,the inter-day precision were 1.2% and 2.0%,respectively.The spike recovery for the total ... |
| Keywords: | Cell digestion high performance liquid chromatography-inductively coupled plasma-mass sepctrometry Chang liver hepatocytes arsenic speciation |
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