Nitric oxide enhances the capacitance of self-assembled,supported bilayer lipid membranes |
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| Institution: | 1. SEG-CEMUC — Department of Mechanical Engineering, University of Coimbra, Portugal;2. Universidade do Porto, Faculdade de Engenharia, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal;3. Centro de Física, Universidade do Minho, 4710-057 Braga, Portugal;4. Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, Ilmenau, Germany;5. Instituto de Plasmas e Fusão Nuclear, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisboa, Portugal;6. Centro de Ciências e Tecnologias Nucleares, Instituto Superior Técnico, Universidade de Lisboa, E.N. 10 (km 139,7), 2695-066 Bobadela LRS, Portugal;7. Institut FEMTO-ST, UMR 6174 (CNRS, UFC, ENSMM, UTBM), 15B, Avenue des Montboucons, 25030 Besancon Cedex, France;1. Institute of Condensed Matter Physics (ICMP), Swiss Federal Institute of Technology Lausanne (EPFL), CH-1015 Lausanne, Switzerland;2. IHI Ionbond AG, CH-4600 Olten, Switzerland |
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| Abstract: | The effect of nitric oxide (NO) at biologically relevant concentrations on the electrochemical features of the membrane was investigated by cyclic voltammetry (CV) at self-assembled, stainless steel supported lipid bilayer membranes (s-BLMs) using a three-electrode system. The results showed that the membrane capacitance (Cm) of s-BLMs was dramatically enhanced by the presence of increasing NO concentration from 0 to 70 μM. For comparison, fullerene C60 doped s-BLMs (C60@s-BLMs) was also studied. The Cm of C60@s-BLMs increased with NO concentration from 0 to 16 μM and gradually reached a plateau value when NO concentration was over 16 μM. We concluded that (i) NO accumulated inside lipid bilayer increases the Cm of s-BLMs, and (ii) C60 inside s-BLMs changes the dielectric constant of lipid bilayer, thus reducing the effect of NO on the Cm of C60@s-BLMs. This novel self-assembled lipid modified probe provides a simple yet interesting model to study the effect of NO on the electrical conductance of the membrane. |
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