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Inner wall coating of micro-LC columns. Wall effects in LC
Authors:M Verzele  C Dewaele  M De Weerdt  S Abbott
Abstract:An elastic inner wall coating in the fused silica capillaries used for Micro-LC (LC on packed fused silica capillary columns) stabilizes the packed bed and thus increases column efficiency and life expectancy. Probably the particles of packing material are partly forced into the elastic polymer layer which thus holds the packing in position. Bonded polymers of very different chemical polarity can be used with equally good results. Variation of the coating layer thickness shows that there is an optimum value around 0.3 μm. A discussion of various wall effects in LC columns is presented. The i.d. of the columns is a most important parameter in this respect.
Keywords:Micro-LC  Wall coating  Wall effects
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