Protein immobilization capacity and covalent binding coverage of pulsed plasma polymer surfaces |
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Authors: | Yongbai Yin Daniel Bax David R. McKenzie Marcela M.M. Bilek |
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Affiliation: | Applied & Plasma Physics Group, Physics School, University of Sydney, NSW 2006, Australia |
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Abstract: | Three carbon surfaces were deposited using pulsed plasma enhanced chemical vapour deposition method: a low and a high nitrogen-containing plasma polymer surfaces and a diamond-like carbon surface. The surfaces were analysed using both X-ray photoelectron spectroscopy (XPS) technique and the enzyme-linked immunosorbent assay (ELISA) method combining with sodium dodecyl sulphate (SDS) cleaning to investigate the capacity and covalent binding of the immobilized proteins. A good correlation was found on quantification of remaining protein after SDS cleaning using the ELISA method and the XPS technique. All surfaces had similar initial capacity of protein attachment but with large different resistance to SDS cleaning. The analysis showed that the high nitrogen-containing plasma polymer was the best biocompatible material due to its highest resistance to SDS cleaning, i.e. with the highest quantity (∼80%) of proteins bound covalently. |
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Keywords: | Plasma polymerized surface Protein immobilization X-ray photoelectron spectroscopy Enzyme-linked immunosorbent assay SDS cleaning Tropoelastin |
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