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Spontaneously Blinking Rhodamine Dyes for Single-Molecule Localization Microscopy |
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| Authors: | Prof Weijie Chi Dr Davin Tan Prof Qinglong Qiao Prof Zhaochao Xu Prof Xiaogang Liu |
| Institution: | 1. Collaborative Innovation Center of One Health, School of Science, Hainan University, Renmin Road 58, Haikou, 570228 P. R. China
Fluorescence Research Group, Singapore University of Technology and Design, 8 Somapah Road, 487372 Singapore, Singapore;2. Fluorescence Research Group, Singapore University of Technology and Design, 8 Somapah Road, 487372 Singapore, Singapore;3. CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023 China |
| Abstract: | Single-molecule localization microscopy (SMLM) has found extensive applications in various fields of biology and chemistry. As a vital component of SMLM, fluorophores play an essential role in obtaining super-resolution fluorescence images. Recent research on spontaneously blinking fluorophores has greatly simplified the experimental setups and extended the imaging duration of SMLM. To support this crucial development, this review provides a comprehensive overview of the development of spontaneously blinking rhodamines from 2014 to 2023, as well as the key mechanistic aspects of intramolecular spirocyclization reactions. We hope that by offering insightful design guidelines, this review will contribute to accelerating the advancement of super-resolution imaging technologies. |
| Keywords: | Rhodamine Dyes Single-Molecule Localization Microscopy Spirocyclization Equilibrium Spontaneously Blinking Fluorophores |