Covalent Organic Framework Based Crosslinked Porous Microcapsules for Enzymatic Catalysis |
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Authors: | Mengchu Feng Ziru Niu Chunyan Xing Yehao Jin Prof. Dr. Xiao Feng Dr. Yuanyuan Zhang Prof. Dr. Bo Wang |
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Affiliation: | Frontiers Science Center for High Energy Material, Advanced Technology Research Institute (Jinan), Key Laboratory of Cluster Science (Ministry of Education), Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion Materials, Advanced Research Institute of Multidisciplinary Science, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 100081 P. R. China |
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Abstract: | The design of porous microcapsules with selective mass transfer and mechanical robustness for enzyme encapsulation is highly desired for biocatalysis, yet the construction remains challenging. Herein, we report the facile fabrication of porous microcapsules by assembling covalent organic framework (COF) spheres at the interfaces of emulsion droplets followed by interparticle crosslinking. The COF microcapsules could offer an enclosed aqueous environment for enzymes, with size-selective porous shells that allow for the fast diffusion of substrates and products while excluding larger molecules such as protease. Crosslinking of COF spheres not only enhances the structural stability of capsules but also imparts enrichment effects. The enzymes encased in the COF microcapsules show enhanced activity and durability in organic media as verified in both batch reaction and continuous-flow reaction. The COF microcapsules offer a promising platform for the encapsulation of biomacromolecules. |
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Keywords: | Covalent Organic Frameworks Enzyme Encapsulation Microcapsules Porous Materials |
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