| Determination of Amino Acids in Single Human Lymphocytes after On-capillary Derivatization by Capillary Zone Electrophoresis with Electrochemical Detection |
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| 引用本文： | Qian Feng WENG,Wen Rui JIN* School of Chemistry and Chemical Engineering,Shandong University,Jinan 250100. Determination of Amino Acids in Single Human Lymphocytes after On-capillary Derivatization by Capillary Zone Electrophoresis with Electrochemical Detection[J]. 中国化学快报, 2002, 13(10) |
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| 作者姓名： | Qian Feng WENG Wen Rui JIN* School of Chemistry and Chemical Engineering Shandong University Jinan 250100 |
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| 作者单位： | Qian Feng WENG，Wen Rui JIN* School of Chemistry and Chemical Engineering，Shandong University，Jinan 250100 |
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| 摘 要： | Sensitive and selective methods for the detection of amino acids (AAs) in single cells are of increasing importance. Capillary zone electrophoresis (CZE) with electrochemical detection (ED) is suitable for analysis of electroactive contents in single cells. However, most native AAs have no electroactivity. Derivatization with an electroactive tag is an attractive method. An on-capillary derivatization scheme was reported1. Naphtha- lene-2, 3-dicarboxaldehyde (NDA) can react with pri…
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Determination of Amino Acids in Single Human Lymphocytes after On-capillary Derivatization by Capillary Zone Electrophoresis with Electrochemical Detection |
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| Qian Feng WENG,Wen Rui JIN. Determination of Amino Acids in Single Human Lymphocytes after On-capillary Derivatization by Capillary Zone Electrophoresis with Electrochemical Detection[J]. Chinese Chemical Letters, 2002, 13(10) |
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| Authors: | Qian Feng WENG Wen Rui JIN |
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| Affiliation: | School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100 |
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| Abstract: | Amino acids in individual human lymphocytes were determined by capillary zone electrophoresis with electrochemical detection after on-capillary derivatization. In order to inject cells easily, a cell injector was designed. Four amino acids (serine, alanine, taurine, and glycine) in single human lymphocytes have been identified. Quantitation has been accomplished through the use of calibration curves. |
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| Keywords: | Capillary electrophoresis electrochemical detection amino acid lymphocyte. |
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