| 螺旋藻抗氧化酶系统对干燥胁迫的响应 |
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| 引用本文： | 陈思嘉,陈填烽,杨芳,郑文杰,白燕.螺旋藻抗氧化酶系统对干燥胁迫的响应[J].暨南大学学报,2006,27(1):123-128. |
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| 作者姓名： | 陈思嘉 陈填烽 杨芳 郑文杰 白燕 |
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| 作者单位： | 暨南大学,化学系,广东,广州,510632;暨南大学,化学系,广东,广州,510632;暨南大学,水生生物研究所,广东,广州,510632 |
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| 基金项目： | 教育部重点研究项目;广东省广州市科技攻关项目 |
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| 摘 要： | 对极大螺旋藻(Spirulina maxima)进行常温脱水处理,研究干燥胁迫对螺旋藻抗氧化酶活性的影响.结果表明:螺旋藻超氧化物歧化酶(SOD)对细胞脱水反应不敏感,脱水过程中其活性保持不变,过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)能很快对干燥胁迫作出响应,两者活性均上升1倍左右,而过氧化物酶(POD)活性呈下降趋势.干燥胁迫30 h后各抗氧化酶活性均下降,其中POD失活.丙二醛(MDA)质量摩尔浓度在整个胁迫过程中波动较小,脱水过程中略有下降,干燥胁迫30 h后又有所上升.螺旋藻的抗氧化酶系统能抵御细胞脱水所引起的氧化伤害,其中CAT和APX的活性变化幅度可作为检验螺旋藻脱水耐受力的敏感性指标.
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| 关 键 词： | 螺旋藻 抗氧化酶 干燥胁迫 脱水耐受力 |
| 文章编号： | 1000-9965(2006)01-0123-06 |
| 修稿时间： | 2005年4月22日 |
Response of antioxidase system in Spirulina maxima to desiccation stress |
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| CHEN Si-jia,CHEN Tian-feng,YANG Fang,ZHENG Wen-jie,BAI Yan.Response of antioxidase system in Spirulina maxima to desiccation stress[J].Journal of Jinan University(Natural Science & Medicine Edition),2006,27(1):123-128. |
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| Authors: | CHEN Si-jia CHEN Tian-feng YANG Fang ZHENG Wen-jie BAI Yan |
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| Abstract: | The effects of desiccation stress on antioxidase system of Spirulina maxima were studied by dehydration treatment under room temperature.The results show that SOD of S.maxima is not sensitive to dehydration,and the activity of SOD keep unchanged during dehydration.CAT and APX have fast response to desiccation stress with the activity increasing to twice as high as control.However,the activity of POD decreases during dehydration.All of the antioxidase activity drop after 30h's desiccation stress.The content of MDA changes slightly,which drops during dehydration and increases after 30h.The antioxidase system of S.maxima is able to defend against oxidation damage induced by dehydration.The activity of CAT and APX are possible sensitive indicators for the dehydration tolerance of spirulina. |
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| Keywords: | Spirulina maxima antioxidase desiccation stress dehydration tolerance |
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